Monthly Archives: August 2017

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Differential effects of rapalogues, dual kinase inhibitors on human ovarian carcinoma cells in vitro

Relative expression of mTOR, DEPTOR, rictor and raptor were assessed by quantitative PCR (Q-PCR) on an xxpress® (BJS Biotechnologies, Middlesex, UK) thermal cycler using Kapa SYBR Fast Universal Mastermix (Kapa Biosystems, MA, USA). Abstract: Ovarian cancer is the second most common [...]

xxpress RT-qPCR to Analyse the Quality and Quantity of cDNA

A recent article by the Queensland University of Technology investigated candidate genes for facilitating freshwater adaptation in palaemonid prawns. These genes were identified and their expression validated in the model species M. koombooloomba. Abstract Background: The endemic Australian freshwater prawn, Macrobrachium koombooloomba, provides a [...]

Oxytocin Reverses the Actions of Cortisol in Human Ovarian Carcinoma Cells in Vitro

Relative expression of the genes of interest was assessed by quantitative PCR (Q-PCR) on an ABI7400 instrument (Applied Biosystems) and xxpress® (BJS Biotechnologies). Abstract The journey patients with ovarian cancer travel from non-specific symptoms causing delayed diagnosis through surgery and chemotherapy, [...]

Development of a Fast and Low-Cost qPCR Assay for Diagnosis of Acute Gas Pharyngitis

The qPCR was carried out using four different Real-Time PCR system: Biorad CFX Connect (Bio-Rad Laboratories, USA), LightCycler 480 (Roche Applied Science, USA), StepOne Plus (Life Technologies, USA) and Xxpress (BJS Biotechnologies, UK). Background: Group A streptococci (GAS) are the [...]

The Avian Influenza Virus: Distinguishing its High and Low Threat Pathotypes

Avian Influenza Virus (AIV) Pathogenicity refers to the ability of an organism to harm its host by causing disease. The avian influenza virus, subtype H7, exists as one of two pathotypes, highly pathogenic (HPAIV) and low pathogenic (LPAIV). Even LPAIV carries a [...]

Rosalind Franklin

Last week marked the anniversary of the birth of Rosalind Franklin, born 25th July 1920.   Franklin worked as a chemist and x-ray crystallographer, contributing to many groundbreaking developments and greatly enhancing scientific understanding. This post looks back at some [...]

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