PCR Types Performed by the xxpress® Thermal Cycler

/PCR Types Performed by the xxpress® Thermal Cycler

PCR Types Performed by the xxpress® Thermal Cycler

To read the white paper, click here.

Introduction

Since the polymerase chain reaction (PCR) was first described by Kary Mullis in 1983, the process has been greatly developed and modified, originating a new range of PCR techniques and applications. This white paper investigates the wide variety of techniques that utilise PCR and demonstrates how the speed and accuracy of the xxpress® qPCR thermal cycler makes it ideal for performing these methods.

References

Dorak, M. (2006). Real-time PCR. Taylor & Francis Group: Abingdon. 

Jing, C., Wang, L., Wang, T., Zhang, J., Dong, W., Zhang, X. (2011). Amplification of deoxyribonucleic acid (DNA) fragment using two-step polymerase chain reaction (PCR). African Journal of Biotechnology, 10(15): 2838-2843.

Kubista, M. et al. (2006). The real-time polymerase chain reaction. Molecular Aspects of Medicine, 27: 95-125.

Logan J., Edwards, K., Saunders, N. (2009). Real-time PCR. Current technology and Applications. Caister Academic Press: Norfolk.

Navarro, E., Serrano-Heras, G., Castaño, M.J., Solera, J. (2015). Real-time PCR detection chemistry. Clinica Chimica Acta, 439: 231-250.

Słomka, M., Sobalska-Kwapis, M., Wachulec, M., Bartosz, G., Strapagiel, D. (2017).  High Resolution Melting (HRM) for High-Throughput Genotyping—Limitations and Caveats in Practical Case Studies. International Journal of Molecular Sciences, 18(2316): 1-21.

Wittwer, C. (2009). High-Resolution DNA Melting Analysis: Advancements and Limitations. Human Mutation, 30(6): 857-859.

 

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