To read the white paper, click here.
Since the polymerase chain reaction (PCR) was first described by Kary Mullis in 1983, the process has been greatly developed and modified, originating a new range of PCR techniques and applications. This white paper investigates the wide variety of techniques that utilise PCR and demonstrates how the speed and accuracy of the xxpress® qPCR thermal cycler makes it ideal for performing these methods.
Jing, C., Wang, L., Wang, T., Zhang, J., Dong, W., Zhang, X. (2011). Amplification of deoxyribonucleic acid (DNA) fragment using two-step polymerase chain reaction (PCR). African Journal of Biotechnology, 10(15): 2838-2843.
Słomka, M., Sobalska-Kwapis, M., Wachulec, M., Bartosz, G., Strapagiel, D. (2017). High Resolution Melting (HRM) for High-Throughput Genotyping—Limitations and Caveats in Practical Case Studies. International Journal of Molecular Sciences, 18(2316): 1-21.