Over the last few years, qPCR has become a reference technique in the field of molecular biology due to its simplicity, sensitivity and specificity, which is reflected in the high number of publications using this method. However, qPCR is still limited by the speed of the process, taking up to 2 hours to perform a 40cycle qPCR analysis on a typical peltier-based device. The xxpress® qPCR thermal cycler provides a solution to this problem and is able to complete a whole reaction in as little as 10 minutes.
To continue reading this white paper, click the link here.
Run 1 – 3.3oC/s ramp rate – Recommended thermal protocol
Run 2 – xxpress ramp rate – Recommended thermal protocol
Run 3 – xxpress ramp rate – Optimized thermal protocol