A high-resolution melt (HRM) is a powerful, post-PCR analysis technique, used to identify the DNA products present. This method has a wide variety of uses from determining the specificity of a PCR reaction to detecting mutations and polymorphisms. It is [...]
The polymerase chain reaction (PCR), sometimes described as ‘molecular photocopying’, is a laboratory technique that allows researchers and technicians to amplify DNA or RNA from as little as a single strand. The technology was first created in 1983 by Biochemist [...]
The Minimum Information for Publication of Quantitative Real-Time PCR Experiments. The MIQE guidelines were introduced to reduce the lack of standardisation in performing and evaluating qPCR experiments. Previously, a lack of consensus was causing several issues within the field. In [...]
A recent article, by Maggie Koreth-Baker for Nautilus, details the story of the Polymerase Chain Reaction "from a Pink Squiggle to the Human Genome Project". The article details the discovery of the thermophilic bacterium, Thermus Aquaticus, from which heat-resistant Taq [...]
In 2013, the polymerase chain reaction (PCR) turned 30. To celebrate these, here's a roundup of articles celebrating PCR on the web and from our archive. Source: steve-spicer.com Start with PCR 101: An introduction to PCR, and then [...]
These days, few people are seen without a smartphone, whether it's an Android or an iPhone, and their computing power and storage capabilities are increasing. The numbers of apps for smartphones are increasing, and these are starting to make their [...]
The polymerase chain reaction (PCR), sometimes described as 'molecular photocopying', is a laboratory technique that allows researchers and technicians to replicate and amplify DNA or RNA from small or damaged samples, even down to a single strand. The technology was [...]
The polymerase chain reaction (PCR) has a wealth of applications in research, but perhaps one of its most challenging roles in single cell analysis, amplifying the DNA from just a single cell, or a very small sample of cells. [...]
Mutation analysis is the process of finding known and unknown mutations in DNA and RNA, and PCR (polymerase chain reaction) is used as part of the method. Source: steve-spicer.com In the clinic, mutation analysis can be used to [...]
Happy 60th birthday to DNA on 23 April 2013, DNA Day! Today marks the 60th anniversary of the publication of papers in Nature on the discovery of the structure of DNA in 1953. A lot has happened since the publication [...]
