Fundamentals of PCR

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Happy DNA Day: Celebrate the 60th anniversary of the discovery of the structure of DNA

Happy 60th birthday to DNA on 23 April 2013, DNA Day! Today marks the 60th anniversary of the publication of papers in Nature on the discovery of the structure of DNA in 1953. A lot has happened since the publication [...]

Normalisation of samples in gene expression analysis

Reverse transcription PCR (RT-PCR) has been used to quantify mRNA (messenger RNA) in gene expression analysis for many years. Researchers generally use the technique to compare two or more samples, for example looking at cancers at different stages, or comparing [...]

2018-01-19T10:09:59+00:00March 21st, 2013|Categories: All News, Fundamentals of PCR|Tags: , , , |

Applications in PCR: Using PCR to track migration

PCR (polymerase chain reaction) amplification of ancient and modern DNA samples can be used by researchers to track migration of animal and human populations, shedding light on evolution and the movement of ethnic groups across continents. There are many different [...]

PCR helps researchers view cellular functions

Researchers at the US Rensselaer Polytechnic Institute have created a new and very sensitive technique to detect the glycans (sugars) in living organisms. Dubbed glyco-qPCR, this uses quantitative PCR (qPCR; real-time PCR) techniques. The genome (the whole of a cell, [...]

Applications in PCR: PCR in gene expression analysis

Nearly all of the cells in an organism carry the same genetic material and so the same collection of genes, but different cells have different roles and so have different profiles of genes active at any one time. Gene expression [...]

Applications in PCR: PCR in genetic and genome engineering

Genetic and genome engineering is a useful tool for researchers, from producing proteins to understanding disease, and the polymerase chain reaction (PCR) has a vital role supporting this process by cloning the DNA fragments used to modify the genomes of [...]

Applications in PCR: PCR in IVF

PCR (polymerase chain reaction) is used in pre-implantation genetic diagnosis (PGD) in IVF (in vitro fertilisation) to screen the embryo before implantation, avoiding transfer of an embryo carrying a genetic flaw. This has a number of applications: For families at [...]

Working in real time: A focus on qPCR

There are lots of different types of PCR (polymerase chain reaction), with many different applications, so what’s so special about quantitative PCR (qPCR), also known as real time PCR, RT PCR or qRT-PCR? Measuring in real time Whereas conventional PCR [...]

What are the different kinds of PCR?

PCR (polymerase chain reaction) is an amazing tool for use in clinical and diagnostic medicine and research, but there is more than just one kind, all with different applications and levels of sensitivity. This is a whirlwind trip around the [...]

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