PCR in diagnosis

/PCR in diagnosis

Early foetal sex determination using micro-volume of maternal plasma

The presence of cell-free foetal DNA (cffDNA) in maternal plasma was first discovered by Lo et al. in 1997, opening a new range of possibilities for non-invasive prenatal testing. cffDNA represents around 10% of total cell-free DNA in maternal circulation, [...]

Rapid, Accurate Diagnosis of Clostridium difficile Infections

Clostridium difficile infection (CDI) is a symptomatic infectious disease caused by the anaerobic, spore-forming bacterium called Clostridium difficile. Recently CDI has become increasingly prevalent worldwide. The severity of the infection is also on the rise, meaning the requirement for a rapid and accurate method of diagnosis [...]

Effectiveness of qPCR for the Detection of TB

Tuberculosis is an infectious disease caused by the bacteria Mycobacterium tuberculosis. It is the second leading cause of death from an infectious disease and predominantly spread through an airborne route. It continues to be a significant global health issue causing about [...]

The Future of Viral Diagnosis?

A Novel Quantitative PCR Mediated by High-Fidelity DNA Polymerase. Traditional PCR methods can be used for viral diagnosis, however they are required to be highly sensitivity, specific and reproducible for this use. These criteria are largely dependent on the complementarity [...]

Development of a Fast and Low-Cost qPCR Assay for Diagnosis of Acute Gas Pharyngitis

The qPCR was carried out using four different Real-Time PCR system: Biorad CFX Connect (Bio-Rad Laboratories, USA), LightCycler 480 (Roche Applied Science, USA), StepOne Plus (Life Technologies, USA) and Xxpress (BJS Biotechnologies, UK). Background: Group A streptococci (GAS) are the [...]

The Avian Influenza Virus: Distinguishing its High and Low Threat Pathotypes

Avian Influenza Virus (AIV) Pathogenicity refers to the ability of an organism to harm its host by causing disease. The avian influenza virus, subtype H7, exists as one of two pathotypes, highly pathogenic (HPAIV) and low pathogenic (LPAIV). Even LPAIV carries a [...]

xxpress PCR and Prosthetic Joint Infection Diagnosis

The ultra-fast speed of the xxpress qPCR thermal cycler is advantageous for a wide variety of uses. Recently a paper was published in the Journal of Clinical Microbiology detailing the ability of the xxpress to be used for the "timely [...]

Monitoring Transplant Patients for M. hominis Infections

A recent study has revealed the importance of monitoring transplant donors and recipients for Mycoplasma hominis infections. Mycoplasma hominis colonies M. hominis is a species of bacteria able to infect and penetrate the interior of human cells. It is notoriously difficult to test [...]

HRM for the Detection of Important Poxviruses

A high resolution melt (HRM) is a powerful, post-PCR, analysis technique used to identify the DNA products present. First of all PCR is performed with an intercalating dye present. These dyes have the unique property of only binding to double-stranded DNA. [...]

New Zika test approved for CDC use

Zika, Dengue and Chikungunya; 3 viruses alike in their symptoms and their transmission by Aedes mosquitoes. Former PCR-based Zika tests were unable to rule out infections with Dengue or Chikungunya. A trioplex real-time RT-PCR assay has now been developed for the [...]

The xxcloud is our exciting new software release, enabling users to programme and analyse results remotely, from a PC or laptop.

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