PCR in research

/PCR in research

Understanding the Pathogenesis of FCD Type II Using qPCR

Focal Cortical Dysplasia (FCD) is a malformation of cortical development (Kabat and Król, 2012). It is increasingly known as one of the most common causes of pharmaco-resistant epilepsies in both adults and children (Kabat and Król, 2012) (Najm et al, [...]

Differential effects of rapalogues, dual kinase inhibitors on human ovarian carcinoma cells in vitro

Relative expression of mTOR, DEPTOR, rictor and raptor were assessed by quantitative PCR (Q-PCR) on an xxpress® (BJS Biotechnologies, Middlesex, UK) thermal cycler using Kapa SYBR Fast Universal Mastermix (Kapa Biosystems, MA, USA). Abstract: Ovarian cancer is the second most common [...]

xxpress RT-qPCR to Analyse the Quality and Quantity of cDNA

A recent article by the Queensland University of Technology investigated candidate genes for facilitating freshwater adaptation in palaemonid prawns. These genes were identified and their expression validated in the model species M. koombooloomba. Abstract Background: The endemic Australian freshwater prawn, Macrobrachium koombooloomba, provides a [...]

Oxytocin Reverses the Actions of Cortisol in Human Ovarian Carcinoma Cells in Vitro

Relative expression of the genes of interest was assessed by quantitative PCR (Q-PCR) on an ABI7400 instrument (Applied Biosystems) and xxpress® (BJS Biotechnologies). Abstract The journey patients with ovarian cancer travel from non-specific symptoms causing delayed diagnosis through surgery and chemotherapy, [...]

xxpress PCR and Prosthetic Joint Infection Diagnosis

The ultra-fast speed of the xxpress qPCR thermal cycler is advantageous for a wide variety of uses. Recently a paper was published in the Journal of Clinical Microbiology detailing the ability of the xxpress to be used for the "timely [...]

Monitoring Transplant Patients for M. hominis Infections

A recent study has revealed the importance of monitoring transplant donors and recipients for Mycoplasma hominis infections. Mycoplasma hominis colonies M. hominis is a species of bacteria able to infect and penetrate the interior of human cells. It is notoriously difficult to test [...]

Successful completion of BJS Biotechnologies projects at UCL

  File 1 - Successful completion of BJS Biotechnologies projects at UCL

RT-qPCR to Determine the Role of TEX19 in Cancers

Cancer/testis (CT) genes are one of the major groups of germline genes. They encode proteins known as cancer/testis antigens (CTAs), usually limited to the healthy adult testis. These antigens were initially identified by autologous typing, but thanks to PCR technology [...]

World’s First Malaria Vaccine Trial

Ahead of World Malaria Day tomorrow comes an exciting development. The worlds first malaria vaccine has been given the go ahead for large-scale trials in three African countries: Kenya, Ghana and Malawi. These were selected as the initial countries to test the [...]

Detecting Aquatic Organisms Using eDNA

Nuclear or mitochondrial DNA that is released into the environment from an organism is known as environmental DNA or eDNA. The eDNA of a large number of aquatic organisms can be detected in water samples by qPCR, even when at [...]

The xxcloud is our exciting new software release, enabling users to programme and analyse results remotely, from a PC or laptop.


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