qPCR

/qPCR

xxpress® qPCR Thermal Cycler Comparison

Thinking of purchasing a new thermal cycler? There are lots of different factors to take into consideration; the cost involved, the compatibility of different sample formats, cycling speed and thermal uniformity are often regarded as the most important. We've already [...]

Outstanding Return on Investment with the xxpress® qPCR Thermal Cycler

There are many benefits to the xxpress® qPCR thermal cycler, one very attractive quality being the outstanding return on investment (ROI) it provides. This is an important factor for many institutions when looking to invest in a new piece of [...]

Rapid, Accurate Diagnosis of Clostridium difficile Infections

Clostridium difficile infection (CDI) is a symptomatic infectious disease caused by the anaerobic, spore-forming bacterium called Clostridium difficile. Recently CDI has become increasingly prevalent worldwide. The severity of the infection is also on the rise, meaning the requirement for a rapid and accurate method of diagnosis [...]

Effectiveness of qPCR for the Detection of TB

Tuberculosis is an infectious disease caused by the bacteria Mycobacterium tuberculosis. It is the second leading cause of death from an infectious disease and predominantly spread through an airborne route. It continues to be a significant global health issue causing about [...]

The Future of Viral Diagnosis?

A Novel Quantitative PCR Mediated by High-Fidelity DNA Polymerase. Traditional PCR methods can be used for viral diagnosis, however they are required to be highly sensitivity, specific and reproducible for this use. These criteria are largely dependent on the complementarity [...]

Differential effects of rapalogues, dual kinase inhibitors on human ovarian carcinoma cells in vitro

Relative expression of mTOR, DEPTOR, rictor and raptor were assessed by quantitative PCR (Q-PCR) on an xxpress® (BJS Biotechnologies, Middlesex, UK) thermal cycler using Kapa SYBR Fast Universal Mastermix (Kapa Biosystems, MA, USA). Abstract: Ovarian cancer is the second most common [...]

xxpress RT-qPCR to Analyse the Quality and Quantity of cDNA

A recent article by the Queensland University of Technology investigated candidate genes for facilitating freshwater adaptation in palaemonid prawns. These genes were identified and their expression validated in the model species M. koombooloomba. Abstract Background: The endemic Australian freshwater prawn, Macrobrachium koombooloomba, provides a [...]

Oxytocin Reverses the Actions of Cortisol in Human Ovarian Carcinoma Cells in Vitro

Relative expression of the genes of interest was assessed by quantitative PCR (Q-PCR) on an ABI7400 instrument (Applied Biosystems) and xxpress® (BJS Biotechnologies). Abstract The journey patients with ovarian cancer travel from non-specific symptoms causing delayed diagnosis through surgery and chemotherapy, [...]

Development of a Fast and Low-Cost qPCR Assay for Diagnosis of Acute Gas Pharyngitis

The qPCR was carried out using four different Real-Time PCR system: Biorad CFX Connect (Bio-Rad Laboratories, USA), LightCycler 480 (Roche Applied Science, USA), StepOne Plus (Life Technologies, USA) and Xxpress (BJS Biotechnologies, UK). Background: Group A streptococci (GAS) are the [...]

The Avian Influenza Virus: Distinguishing its High and Low Threat Pathotypes

Avian Influenza Virus (AIV) Pathogenicity refers to the ability of an organism to harm its host by causing disease. The avian influenza virus, subtype H7, exists as one of two pathotypes, highly pathogenic (HPAIV) and low pathogenic (LPAIV). Even LPAIV carries a [...]

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