Real time PCR

/Real time PCR

PCR Types Performed by the xxpress® Thermal Cycler

To read the white paper, click here. Introduction Since the polymerase chain reaction (PCR) was first described by Kary Mullis in 1983, the process has been greatly developed and modified, originating a new range of PCR techniques and applications. This [...]

Understanding the Pathogenesis of FCD Type II Using qPCR

Focal Cortical Dysplasia (FCD) is a malformation of cortical development (Kabat and Król, 2012). It is increasingly known as one of the most common causes of pharmaco-resistant epilepsies in both adults and children (Kabat and Król, 2012) (Najm et al, [...]

Ultra-fast qPCR with the xxpress® qPCR Thermal Cycler

Over the last few years, qPCR has become a reference technique in the field of molecular biology due to its simplicity, sensitivity and specificity, which is reflected in the high number of publications using this method. However, qPCR is still [...]

xxpress® qPCR Thermal Cycler Comparison

Thinking of purchasing a new thermal cycler? There are lots of different factors to take into consideration; the cost involved, the compatibility of different sample formats, cycling speed and thermal uniformity are often regarded as the most important. We've already [...]

Rapid, Accurate Diagnosis of Clostridium difficile Infections

Clostridium difficile infection (CDI) is a symptomatic infectious disease caused by the anaerobic, spore-forming bacterium called Clostridium difficile. Recently CDI has become increasingly prevalent worldwide. The severity of the infection is also on the rise, meaning the requirement for a rapid and accurate method of diagnosis [...]

Effectiveness of qPCR for the Detection of TB

Tuberculosis is an infectious disease caused by the bacteria Mycobacterium tuberculosis. It is the second leading cause of death from an infectious disease and predominantly spread through an airborne route. It continues to be a significant global health issue causing about [...]

The Future of Viral Diagnosis?

A Novel Quantitative PCR Mediated by High-Fidelity DNA Polymerase. Traditional PCR methods can be used for viral diagnosis, however they are required to be highly sensitivity, specific and reproducible for this use. These criteria are largely dependent on the complementarity [...]

Differential effects of rapalogues, dual kinase inhibitors on human ovarian carcinoma cells in vitro

Relative expression of mTOR, DEPTOR, rictor and raptor were assessed by quantitative PCR (Q-PCR) on an xxpress® (BJS Biotechnologies, Middlesex, UK) thermal cycler using Kapa SYBR Fast Universal Mastermix (Kapa Biosystems, MA, USA). Abstract: Ovarian cancer is the second most common [...]

xxpress RT-qPCR to Analyse the Quality and Quantity of cDNA

A recent article by the Queensland University of Technology investigated candidate genes for facilitating freshwater adaptation in palaemonid prawns. These genes were identified and their expression validated in the model species M. koombooloomba. Abstract Background: The endemic Australian freshwater prawn, Macrobrachium koombooloomba, provides a [...]

Oxytocin Reverses the Actions of Cortisol in Human Ovarian Carcinoma Cells in Vitro

Relative expression of the genes of interest was assessed by quantitative PCR (Q-PCR) on an ABI7400 instrument (Applied Biosystems) and xxpress® (BJS Biotechnologies). Abstract The journey patients with ovarian cancer travel from non-specific symptoms causing delayed diagnosis through surgery and chemotherapy, [...]

The xxcloud is our exciting new software release, enabling users to programme and analyse results remotely, from a PC or laptop.

VISIT XXCLOUD

Cost Savings CalculatorWhere to buyContact us