xxpress PCR musings

/xxpress PCR musings

High-Resolution Melt with the xxpress® qPCR Thermal Cycler

A high-resolution melt (HRM) is a powerful, post-PCR analysis technique, used to identify the DNA products present. This method has a wide variety of uses from determining the specificity of a PCR reaction to detecting mutations and polymorphisms. It is [...]

Differential effects of rapalogues, dual kinase inhibitors on human ovarian carcinoma cells in vitro

Relative expression of mTOR, DEPTOR, rictor and raptor were assessed by quantitative PCR (Q-PCR) on an xxpress® (BJS Biotechnologies, Middlesex, UK) thermal cycler using Kapa SYBR Fast Universal Mastermix (Kapa Biosystems, MA, USA). Abstract: Ovarian cancer is the second most common [...]

xxpress RT-qPCR to Analyse the Quality and Quantity of cDNA

A recent article by the Queensland University of Technology investigated candidate genes for facilitating freshwater adaptation in palaemonid prawns. These genes were identified and their expression validated in the model species M. koombooloomba. Abstract Background: The endemic Australian freshwater prawn, Macrobrachium koombooloomba, provides a [...]

Oxytocin Reverses the Actions of Cortisol in Human Ovarian Carcinoma Cells in Vitro

Relative expression of the genes of interest was assessed by quantitative PCR (Q-PCR) on an ABI7400 instrument (Applied Biosystems) and xxpress® (BJS Biotechnologies). Abstract The journey patients with ovarian cancer travel from non-specific symptoms causing delayed diagnosis through surgery and chemotherapy, [...]

Development of a Fast and Low-Cost qPCR Assay for Diagnosis of Acute Gas Pharyngitis

The qPCR was carried out using four different Real-Time PCR system: Biorad CFX Connect (Bio-Rad Laboratories, USA), LightCycler 480 (Roche Applied Science, USA), StepOne Plus (Life Technologies, USA) and Xxpress (BJS Biotechnologies, UK). Background: Group A streptococci (GAS) are the [...]

xxsealer SA

BJS Biotechnologies are excited to announce the launch of our new, semi-automated heat sealer for use with the xxplate™ SBS.     The use of a heat sealer is required in order to weld a sealing film to the top of an [...]

xxplate™ SBS

BJS Biotechnologies are pleased to reveal that our new xxplate™ SBS is now available.   In 1996 the Society for Biomolecular Screening (SBS), now known as the Society for Biomolecular Sciences created a standard definition of a microtiter plate. A [...]

xxpress PCR and Prosthetic Joint Infection Diagnosis

The ultra-fast speed of the xxpress qPCR thermal cycler is advantageous for a wide variety of uses. Recently a paper was published in the Journal of Clinical Microbiology detailing the ability of the xxpress to be used for the "timely [...]

Christmas gifts for PCR users

It's that time of the year when you are looking for Christmas gifts for the PCR-using scientist in your life. While of course, the best possible Christmas gift is the fastest and most accurate thermal PCR cycler, xxpress, we thought [...]

2014-12-16T10:23:46+00:00 December 16th, 2014|Categories: All News, PCR news, xxpress PCR musings|Tags: , , , , , , |

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