FAQs

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What are the applications of multiplex Real-Time PCR?

Multiplex Real-Time PCR can be applied to relative quantification experiments where the gene of interest and reference gene are co-amplified in the same reaction. Multiplex Real-Time PCR can also be used for allelic discrimination assays, where two differentially labeled probes [...]

2018-01-22T15:05:02+00:00 December 4th, 2017|

What is multiplex Real-Time PCR?

Multiplex Real-Time PCR is a technique in which multiple target sequences are amplified and detected in a single PCR reaction. Amplified sequences are distinguished by the use of different dyes conjugated to the probes. The number of targets that can [...]

2018-01-22T15:07:01+00:00 December 4th, 2017|

What are the phases of PCR amplification?

There are three phases of PCR amplification: exponential, linear, and plateau. The exponential phase is the first phase of PCR amplification. Reaction components are in excess, there is an exact doubling of product each cycle, and the reaction is specific [...]

2018-01-22T15:12:24+00:00 December 4th, 2017|

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