What are the different starting materials that I can use for Real-Time PCR?

/What are the different starting materials that I can use for Real-Time PCR?

What are the different starting materials that I can use for Real-Time PCR?

2018-01-24T08:48:35+00:00December 4th, 2017|

The starting materials for Real-Time PCR can be RNA, genomic DNA, or plasmid DNA. RNA must be reverse-transcribed into complementary DNA (cDNA) before PCR. More recently, kits have become available that can perform RT-qPCR (RT = reverse transcription) directly from cells without the need for a separate RNA synthesis step.

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