What is melt curve analysis?

/What is melt curve analysis?

What is melt curve analysis?

2018-01-24T08:52:20+00:00 December 4th, 2017|

It is a powerful post-PCR analysis technique used to identify the DNA products present. During PCR reaction dyes are incorporated into the DNA which fluoresce in the presence of double stranded DNA. During a melt the DNA is subjected to a rise in temperature which causes it to denature. As more of the DNA becomes single stranded the fluorescence decreases. A particular DNA product will have an associated temperature at which it denatures and therefore loses its fluorescence.

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