What is multiplex Real-Time PCR?

/What is multiplex Real-Time PCR?

What is multiplex Real-Time PCR?

2018-01-22T15:07:01+00:00 December 4th, 2017|

Multiplex Real-Time PCR is a technique in which multiple target sequences are amplified and detected in a single PCR reaction. Amplified sequences are distinguished by the use of different dyes conjugated to the probes. The number of targets that can be detected in a single reaction is technically limited only by the availability of spectrally distinct dyes and the ability of the Real-Time PCR instrument to effectively excite and detect those dyes. Some advantages of multiplex Real-Time PCR are reduced reagent costs, reduction in sample use, and increased throughput.

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