Real-Time PCR uses various fluorescent detection chemistries that allow you to monitor the PCR reaction as it progresses. The amount of fluorescent signal generated is directly proportional to the amount of DNA being synthesized during the PCR reaction. Data are [...]
It is a powerful post-PCR analysis technique used to identify the DNA products present. During PCR reaction dyes are incorporated into the DNA which fluoresce in the presence of double stranded DNA. During a melt the DNA is subjected to [...]
You can export your data as .csv or RDML files.
The simplest and most commonly used method is the dilution or standard curve method. This method calculates PCR efficiency using the linear regression slope of a dilution series based on the following equation: E = 10(-1/slope) -1 The ideal slope [...]
Ramp rate is adjustable up to 10°C per second, the fastest available in the world.
The xxplates are sealed by welding on a heat film prior to performing the PCR. It is therefore not possible to re-use the xxplates. This would also enable contamination to take place.
The xxpress® centrifuge has a special rotor adapted to accommodate the xxplates.
Are the plates and seals for the xxpress® NGx custom, or can I obtain consumable plates from other vendors?
The xxplates used in the system are custom designed for the xxpress® NGx. Both the xxplates and heat films are only available through BJS Biotechnologies approved distributors, check the website to find your local supplier. The plates are in 24 [...]
xxpress® NGx does not use PMTs or photodiodes, it uses a CCD camera as the detector.
The LEDs are designed to outlast the life of the instrument.