Can I export my xxpress® NGx run data for use in other software packages?
You can export your data as .csv or RDML files.
How do I determine the efficiency of my Real-Time PCR assay?
The simplest and most commonly used method is the dilution or standard curve method. This method calculates PCR efficiency using the linear regression slope of a dilution series based on the following equation: E = 10(-1/slope) -1 The ideal slope [...]
How do I determine if my RNA sample is contaminated with genomic DNA?
In Real-Time RT-qPCR, genomic DNA can potentially be co-amplified during the PCR reaction, contaminating the sample and leading to erroneous results. To determine if an RNA sample is contaminated with genomic DNA it is important to include a no-reverse transcriptase [...]
How do I assess the quality of my RNA sample?
RNA quality is perhaps the most important factor in generating reliable and reproducible Real-Time PCR data. Traditionally, RNA quality was assessed using gel electrophoresis and comparing the 28S and 18S ribosomal RNA bands. Gel electrophoresis is a laborious, time consuming, [...]
How do I quantify my RNA sample?
The most widely used method to quantify RNA is traditional UV spectroscopy. A diluted RNA sample is quantified by measuring its absorbance at 260 nm and 280 nm. The concentration is calculated using the equation: [RNA] ?g/ml = A260 x [...]
