Analysis

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How do I assess the quality of my RNA sample?

RNA quality is perhaps the most important factor in generating reliable and reproducible Real-Time PCR data. Traditionally, RNA quality was assessed using gel electrophoresis and comparing the 28S and 18S ribosomal RNA bands. Gel electrophoresis is a laborious, time consuming, [...]

2018-01-24T08:49:57+00:00December 4th, 2017|

How do I quantify my RNA sample?

The most widely used method to quantify RNA is traditional UV spectroscopy. A diluted RNA sample is quantified by measuring its absorbance at 260 nm and 280 nm. The concentration is calculated using the equation: [RNA] ?g/ml = A260 x [...]

2018-01-24T08:49:41+00:00December 4th, 2017|

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