What are the applications of multiplex Real-Time PCR?

Multiplex Real-Time PCR can be applied to relative quantification experiments where the gene of interest and reference gene are co-amplified in the same reaction. Multiplex Real-Time PCR can also be used for allelic discrimination assays, where two differentially labeled probes [...]

2018-01-22T15:05:02+00:00 December 4th, 2017|

What is melt curve analysis?

It is a powerful post-PCR analysis technique used to identify the DNA products present. During PCR reaction dyes are incorporated into the DNA which fluoresce in the presence of double stranded DNA. During a melt the DNA is subjected to [...]

2018-01-24T08:52:20+00:00 December 4th, 2017|

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