Multiplex Real-Time PCR can be applied to relative quantification experiments where the gene of interest and reference gene are co-amplified in the same reaction. Multiplex Real-Time PCR can also be used for allelic discrimination assays, where two differentially labeled probes [...]
Real-Time PCR has been used in quantification of gene expression, viral quantification, validation of array data, pathogen detection, and allelic discrimination.
It is a powerful post-PCR analysis technique used to identify the DNA products present. During PCR reaction dyes are incorporated into the DNA which fluoresce in the presence of double stranded DNA. During a melt the DNA is subjected to [...]