xxpress® does not use PMTs or photodiodes, it uses a CCD camera as the detector.
The LEDs are designed to outlast the life of the instrument.
My current system requires periodic optical calibration to ensure performance; how does the xxpress® system address this?
The xxpress® optical system components do not move during operation, with the exception of the filter wheel, which moves while measuring the five emission wavelengths for each sample at each cycle. The optical system is calibrated prior to shipment and [...]
The acquisition is done by a CCD camera through the top of the plate and through the optical heat seal.
Yes, the xxpress® system is compatible with the following dyes or their generic equivalents: FAM/SYBR, TAMRA, Texas Red, Cy5, Cy5.5. Channel Excitation (nm) Emission (nm) Example Fluorophores Detected 1 470 513–531 SYBR Green I, FAM 2 530 569-588 TAMRA [...]