Anchor & reporter probes

/Anchor & reporter probes

Anchor & reporter probes

2017-12-05T09:33:27+00:00December 5th, 2017|

Two partnering LightCycler (hybridizing) probes that hybridize on the target sequence in close proximity. The anchor probe (donor) emits fluorescence to excite the reporter probe (acceptor) to initiate FRET (see FRET probes). In allelic discrimination assays, it is important that the reporter probe spans the mutation and has a lower Tm than the anchor probe.

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