Dark quencher

/Dark quencher

Dark quencher

2017-12-05T09:35:29+00:00 December 5th, 2017|

This curve is used in Tm analysis. It has the temperature in the x axis and the negative derivative of fluorescence (F) with respect to temperature (T), shown as dF/dT, on the y axis. The reproducibility of a derivative melting curve is high with a standard deviation of only 0.1 oC between runs.

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