Dynamic range

/Dynamic range

Dynamic range

2017-12-05T09:35:54+00:00December 5th, 2017|

The range of initial template concentrations over which accurate Ct values are obtained. If endogenous control is used for DDCt quantitation method, dynamic ranges of target and control should be comparable. In absolute quantitation, interpolation within this range is accurate but extrapolation beyond the dynamic range should be avoided. The larger the dynamic range, the greater the ability to detect samples with high and low copy number in the same run. See also ABI Publication: Understanding Ct Value for the effect on dynamic range on efficiency calculation.

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