As opposed to quantitative analysis using the data collected during exponential phase of PCR, real-time applications can also be used to collect end-point data for qualitative assays. These are either allelic discrimination assays (genotyping) or absence/presence (minus/plus) assays (pathogen detection). For most reliable end-point assay results, there should be no non-specific amplification and the specificity of the assay should be high (i.e., one nucleotide mismatch should not result in any amplification -this can be best achieved by using MGB or LNA probes).