No template controls (NTC)

/No template controls (NTC)

No template controls (NTC)

2017-12-05T14:07:14+00:00December 5th, 2017|

(NTC, a minus sample control) NTC includes all of the RT-PCR reagents except the RNA template. No product should be synthesized in the NTC or NAC; if a product is amplified, this indicates contamination (fluorescent or PCR products) or presence of genomic DNA in the RNA sample. NTC is not equivalent to H2O controls and H2O controls are not used in qPCR experiments.

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