Quencher

/Quencher

Quencher

2017-12-05T14:09:47+00:00 December 5th, 2017|

The molecule that absorbs the emission of fluorescent reporter when in close vicinity (6 to 10 nucleotides). Commonly used quenchers include TAMRA (fluorescent), and non-fluorescent ones DABCYL and black hole quencher (BHQ) dyes. The quenchers are usually at the 3’ end of a dual-labeled fluorescent probe. Quencher dye is also called acceptor. A quencher’s efficiency increases as the spectral overlap of the reporter dye emission profile and quencher absorption profile increases (highest for BHQ).

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