Rn (normalized reporter signal)

/Rn (normalized reporter signal)

Rn (normalized reporter signal)

2017-12-05T14:13:59+00:00December 5th, 2017|

The fluorescence emission intensity of the reporter dye divided by the fluorescence emission intensity of the passive reference dye. Rn+ is the Rn value of a reaction containing all components, including the template and Rn– is the Rn value of an unreacted sample. The Rn– value can be obtained from the early cycles of a real-time PCR run (those cycles prior to a significant increase in fluorescence), or a reaction that does not contain any template.

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