2017-12-05T14:15:54+00:00December 5th, 2017|

Mathematically calculated slope of standard curve, e.g., the plot of Ct values against logarithm of ten-fold dilutions of target nucleic acid. This slope is used for efficiency calculation. Ideally, the slope should be –3.32 (–3.1 to –3.6), which corresponds to 100% efficiency (precisely 1.0092) or two-fold (precisely, 2.0092) amplification at each cycle. Also called gradient.

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