Standard deviation (SD)

/Standard deviation (SD)

Standard deviation (SD)

2017-12-05T14:16:52+00:00 December 5th, 2017|

Precision of the real-time PCR values (Ct) are assessed by the standard deviation of the Ct values obtained from the replicates. If SD is >0.25, the power to discriminate between a two-fold dilution is less than 95%. If in a ROX requiring instrument, a master mix with lower than required ROX concentration is used, the SD will be large and may be reduced by using an appropriate master mix. See ABI Publication: Understanding Ct Value for more on SD.

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