SYBR® Green I

/SYBR® Green I

SYBR® Green I

2017-12-05T14:17:53+00:00December 5th, 2017|

A fluorogenic minor groove binding dye that emits little fluorescence when in solution but emits a strong fluorescent signal upon binding to double-stranded DNA. It is used as a cheaper alternative in real-time PCR applications. It does not bind to ssDNA but because of the lack of sequence specificity it binds to any dsDNA product. Its use usually requires melting curve analysis to assure specificity of the results (and if multiplexing is attempted). Similar dsDNA-binding fluorescent dyes developed for high-resolution melting analysis include EvaGreen and LCGreen (see HRM Overview). See also Morrison, 1998 and How it Works.

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