Clostridium difficile infection (CDI) is a symptomatic infectious disease caused by the anaerobic, spore-forming bacterium called Clostridium difficile. Recently CDI has become increasingly prevalent worldwide. The severity of the infection is also on the rise, meaning the requirement for a rapid and accurate method of diagnosis [...]
A high-resolution melt (HRM) is a powerful, post-PCR analysis technique, used to identify the DNA products present. This method has a wide variety of uses from determining the specificity of a PCR reaction to detecting mutations and polymorphisms. It is [...]
Tuberculosis is an infectious disease caused by the bacteria Mycobacterium tuberculosis. It is the second leading cause of death from an infectious disease and predominantly spread through an airborne route. It continues to be a significant global health issue causing about [...]
Biology Week promotes the important and fascinating world of Bioscience, getting people of all ages and knowledge levels participating in fun and interesting life science activities. Biology week is now in its 6th year and has grown in popularity. Events [...]
A Novel Quantitative PCR Mediated by High-Fidelity DNA Polymerase. Traditional PCR methods can be used for viral diagnosis, however they are required to be highly sensitivity, specific and reproducible for this use. These criteria are largely dependent on the complementarity [...]
The polymerase chain reaction (PCR), sometimes described as ‘molecular photocopying’, is a laboratory technique that allows researchers and technicians to amplify DNA or RNA from as little as a single strand. The technology was first created in 1983 by Biochemist [...]
Relative expression of mTOR, DEPTOR, rictor and raptor were assessed by quantitative PCR (Q-PCR) on an xxpress® (BJS Biotechnologies, Middlesex, UK) thermal cycler using Kapa SYBR Fast Universal Mastermix (Kapa Biosystems, MA, USA). Abstract: Ovarian cancer is the second most common [...]
A recent article by the Queensland University of Technology investigated candidate genes for facilitating freshwater adaptation in palaemonid prawns. These genes were identified and their expression validated in the model species M. koombooloomba. Abstract Background: The endemic Australian freshwater prawn, Macrobrachium koombooloomba, provides a [...]
Relative expression of the genes of interest was assessed by quantitative PCR (Q-PCR) on an ABI7400 instrument (Applied Biosystems) and xxpress® (BJS Biotechnologies). Abstract The journey patients with ovarian cancer travel from non-specific symptoms causing delayed diagnosis through surgery and chemotherapy, [...]
The qPCR was carried out using four different Real-Time PCR system: Biorad CFX Connect (Bio-Rad Laboratories, USA), LightCycler 480 (Roche Applied Science, USA), StepOne Plus (Life Technologies, USA) and Xxpress (BJS Biotechnologies, UK). Background: Group A streptococci (GAS) are the [...]
