DNA

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High-Resolution Melt with the xxpress® qPCR Thermal Cycler

A high-resolution melt (HRM) is a powerful, post-PCR analysis technique, used to identify the DNA products present. This method has a wide variety of uses from determining the specificity of a PCR reaction to detecting mutations and polymorphisms. It is [...]

Effectiveness of qPCR for the Detection of TB

Tuberculosis is an infectious disease caused by the bacteria Mycobacterium tuberculosis. It is the second leading cause of death from an infectious disease and predominantly spread through an airborne route. It continues to be a significant global health issue causing about [...]

Polymerase Chain Reaction: The Past, Present and Future

The polymerase chain reaction (PCR), sometimes described as ‘molecular photocopying’, is a laboratory technique that allows researchers and technicians to amplify DNA or RNA from as little as a single strand. The technology was first created in 1983 by Biochemist [...]

xxpress RT-qPCR to Analyse the Quality and Quantity of cDNA

A recent article by the Queensland University of Technology investigated candidate genes for facilitating freshwater adaptation in palaemonid prawns. These genes were identified and their expression validated in the model species M. koombooloomba. Abstract Background: The endemic Australian freshwater prawn, Macrobrachium koombooloomba, provides a [...]

Rosalind Franklin

Last week marked the anniversary of the birth of Rosalind Franklin, born 25th July 1920.   Franklin worked as a chemist and x-ray crystallographer, contributing to many groundbreaking developments and greatly enhancing scientific understanding. This post looks back at some [...]

xxsealer SA

BJS Biotechnologies are excited to announce the launch of our new, semi-automated heat sealer for use with the xxplate™ SBS.     The use of a heat sealer is required in order to weld a sealing film to the top of an [...]

Detecting Aquatic Organisms Using eDNA

Nuclear or mitochondrial DNA that is released into the environment from an organism is known as environmental DNA or eDNA. The eDNA of a large number of aquatic organisms can be detected in water samples by qPCR, even when at [...]

Groundbreaking Gene Therapy for Retinitis Pigmentosa

Retinitis Pigmentosa (RP) is an x-linked, inherited disease that causes the progressive degeneration of rod photoreceptors in the retina. This disease affects ~15,000 people in Britain and slowly leads to an irreversible form of blindness. There are several genes which [...]

Design of a Synthetic Yeast Genome

A multidisciplinary team at The Department of Biomedical Engineering, Johns Hopkins University, have designed a synthetic eukaryotic genome. The project, known as Sc2.0, resulted in a highly modified Saccharomyces cerevisiae genome which was ~8% smaller, with 1.1 megabases inserted, deleted or altered. [...]

HRM for the Detection of Important Poxviruses

A high resolution melt (HRM) is a powerful, post-PCR, analysis technique used to identify the DNA products present. First of all PCR is performed with an intercalating dye present. These dyes have the unique property of only binding to double-stranded DNA. [...]

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