genome

/Tag: genome

Design of a Synthetic Yeast Genome

A multidisciplinary team at The Department of Biomedical Engineering, Johns Hopkins University, have designed a synthetic eukaryotic genome. The project, known as Sc2.0, resulted in a highly modified Saccharomyces cerevisiae genome which was ~8% smaller, with 1.1 megabases inserted, deleted or altered. [...]

The 6-letter genetic code

A collaborative project by The Scripps Research Institute, Centre National de la Recherche Scientifique and Henan Normal University has seen a stable expansion of the genetic alphabet. Their paper has now been published in PNAS detailing the creation of a semisynthetic organism with a 6-letter [...]

Zebrafish and PCR confirm feasibility of high-throughput editing

CRISPR/Cas9 genome editing is widely used as a tool in genetic studies. Researchers looking at zebrafish have combined this technique with the polymerase chain reaction (PCR) to understand more about the functions of genes in humans. The research is published [...]

The long game: Bringing together PCR and big data

A team from University of Idaho and Oberlin College has developed an approach that uses long-range PCR (also known as long PCR) to generate templates from any DNA source for next-generation sequencing, creating large data sets. As an example, the [...]

PCR helps researchers view cellular functions

Researchers at the US Rensselaer Polytechnic Institute have created a new and very sensitive technique to detect the glycans (sugars) in living organisms. Dubbed glyco-qPCR, this uses quantitative PCR (qPCR; real-time PCR) techniques. The genome (the whole of a cell, [...]

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