PCR

/Tag:PCR

Early foetal sex determination using micro-volume of maternal plasma

The presence of cell-free foetal DNA (cffDNA) in maternal plasma was first discovered by Lo et al. in 1997, opening a new range of possibilities for non-invasive prenatal testing. cffDNA represents around 10% of total cell-free DNA in maternal circulation, [...]

Ultra-fast qPCR with the xxpress® qPCR Thermal Cycler

Over the last few years, qPCR has become a reference technique in the field of molecular biology due to its simplicity, sensitivity and specificity, which is reflected in the high number of publications using this method. However, qPCR is still [...]

xxpress® qPCR Thermal Cycler Comparison

Thinking of purchasing a new thermal cycler? There are lots of different factors to take into consideration; the cost involved, the compatibility of different sample formats, cycling speed and thermal uniformity are often regarded as the most important. We've already [...]

Outstanding Return on Investment with the xxpress® qPCR Thermal Cycler

There are many benefits to the xxpress® qPCR thermal cycler, one very attractive quality being the outstanding return on investment (ROI) it provides. This is an important factor for many institutions when looking to invest in a new piece of [...]

Rapid, Accurate Diagnosis of Clostridium difficile Infections

Clostridium difficile infection (CDI) is a symptomatic infectious disease caused by the anaerobic, spore-forming bacterium called Clostridium difficile. Recently CDI has become increasingly prevalent worldwide. The severity of the infection is also on the rise, meaning the requirement for a rapid and accurate method of diagnosis [...]

High-Resolution Melt with the xxpress® qPCR Thermal Cycler

A high-resolution melt (HRM) is a powerful, post-PCR analysis technique, used to identify the DNA products present. This method has a wide variety of uses from determining the specificity of a PCR reaction to detecting mutations and polymorphisms. It is [...]

Effectiveness of qPCR for the Detection of TB

Tuberculosis is an infectious disease caused by the bacteria Mycobacterium tuberculosis. It is the second leading cause of death from an infectious disease and predominantly spread through an airborne route. It continues to be a significant global health issue causing about [...]

Polymerase Chain Reaction: The Past, Present and Future

The polymerase chain reaction (PCR), sometimes described as ‘molecular photocopying’, is a laboratory technique that allows researchers and technicians to amplify DNA or RNA from as little as a single strand. The technology was first created in 1983 by Biochemist [...]

Differential effects of rapalogues, dual kinase inhibitors on human ovarian carcinoma cells in vitro

Relative expression of mTOR, DEPTOR, rictor and raptor were assessed by quantitative PCR (Q-PCR) on an xxpress® (BJS Biotechnologies, Middlesex, UK) thermal cycler using Kapa SYBR Fast Universal Mastermix (Kapa Biosystems, MA, USA). Abstract: Ovarian cancer is the second most common [...]

xxpress RT-qPCR to Analyse the Quality and Quantity of cDNA

A recent article by the Queensland University of Technology investigated candidate genes for facilitating freshwater adaptation in palaemonid prawns. These genes were identified and their expression validated in the model species M. koombooloomba. Abstract Background: The endemic Australian freshwater prawn, Macrobrachium koombooloomba, provides a [...]

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