qPCR

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Polymerase Chain Reaction: The Past, Present and Future

The polymerase chain reaction (PCR), sometimes described as ‘molecular photocopying’, is a laboratory technique that allows researchers and technicians to amplify DNA or RNA from as little as a single strand. The technology was first created in 1983 by Biochemist [...]

Differential effects of rapalogues, dual kinase inhibitors on human ovarian carcinoma cells in vitro

Relative expression of mTOR, DEPTOR, rictor and raptor were assessed by quantitative PCR (Q-PCR) on an xxpress® (BJS Biotechnologies, Middlesex, UK) thermal cycler using Kapa SYBR Fast Universal Mastermix (Kapa Biosystems, MA, USA). Abstract: Ovarian cancer is the second most common [...]

Oxytocin Reverses the Actions of Cortisol in Human Ovarian Carcinoma Cells in Vitro

Relative expression of the genes of interest was assessed by quantitative PCR (Q-PCR) on an ABI7400 instrument (Applied Biosystems) and xxpress® (BJS Biotechnologies). Abstract The journey patients with ovarian cancer travel from non-specific symptoms causing delayed diagnosis through surgery and chemotherapy, [...]

The Avian Influenza Virus: Distinguishing its High and Low Threat Pathotypes

Avian Influenza Virus (AIV) Pathogenicity refers to the ability of an organism to harm its host by causing disease. The avian influenza virus, subtype H7, exists as one of two pathotypes, highly pathogenic (HPAIV) and low pathogenic (LPAIV). Even LPAIV carries a [...]

xxsealer SA

BJS Biotechnologies are excited to announce the launch of our new, semi-automated heat sealer for use with the xxplate™ SBS.     The use of a heat sealer is required in order to weld a sealing film to the top of an [...]

xxplate™ SBS

BJS Biotechnologies are pleased to reveal that our new xxplate™ SBS is now available.   In 1996 the Society for Biomolecular Screening (SBS), now known as the Society for Biomolecular Sciences created a standard definition of a microtiter plate. A [...]

xxpress PCR and Prosthetic Joint Infection Diagnosis

The ultra-fast speed of the xxpress qPCR thermal cycler is advantageous for a wide variety of uses. Recently a paper was published in the Journal of Clinical Microbiology detailing the ability of the xxpress to be used for the "timely [...]

Testing Water Samples for Legionella

Evaluation of Legionella qPCR against traditional culture for routine and public health testing of water samples. Legionella is a pathogenic, gram-negative bacteria responsible for causing Legionnaires' disease, a potentially fatal form of pneumonia, for which there is currently no vaccine. The bacteria [...]

Standard Dilution Series with the xxpress® qPCR Thermal Cycler

The success of a qPCR reaction is evaluated using a standard dilution series, the data from which is used to calculate the reaction efficiency and R2 values. Efficiency can be defined as the increase in amplicon per cycle, whilst R2 [...]

Detecting Aquatic Organisms Using eDNA

Nuclear or mitochondrial DNA that is released into the environment from an organism is known as environmental DNA or eDNA. The eDNA of a large number of aquatic organisms can be detected in water samples by qPCR, even when at [...]

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