quantitative PCR

/Tag:quantitative PCR

The Future of Viral Diagnosis?

A Novel Quantitative PCR Mediated by High-Fidelity DNA Polymerase. Traditional PCR methods can be used for viral diagnosis, however they are required to be highly sensitivity, specific and reproducible for this use. These criteria are largely dependent on the complementarity [...]

Polymerase Chain Reaction: The Past, Present and Future

The polymerase chain reaction (PCR), sometimes described as ‘molecular photocopying’, is a laboratory technique that allows researchers and technicians to amplify DNA or RNA from as little as a single strand. The technology was first created in 1983 by Biochemist [...]

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Leukaemia monitoring supported by PCR

The polymerase chain reaction (PCR) has a role in monitoring responses to treatment of children with acute lymphoblastic leukaemia (ALL), according to a report in the Journal of Clinical Oncology. Acute lymphoblastic leukaemia is the most common paediatric cancer. Being [...]

qPCR can help to diagnose depression

Quantitative PCR has played a part in the development of what could be the first objective test for major depressive disorders (MDD) in adults, and one that could predict who might benefit from cognitive behavioural therapy (CBT). Depression is generally [...]

Using qPCR to learn more about chikungunya

Researchers at the University Malaya Medical Centre in Kuala Lumpur, Malaysia, are using quantitative polymerase chain reaction (qPCR; also known as real time PCR, RT PCR or qRT-PCR) to understand more about how the chikungunya virus replicates. This could lead [...]

PCR, from the past to the future

The polymerase chain reaction (PCR), sometimes described as 'molecular photocopying', is a laboratory technique that allows researchers and technicians to replicate and amplify DNA or RNA from small or damaged samples, even down to a single strand. The technology was [...]

PCR helps researchers view cellular functions

Researchers at the US Rensselaer Polytechnic Institute have created a new and very sensitive technique to detect the glycans (sugars) in living organisms. Dubbed glyco-qPCR, this uses quantitative PCR (qPCR; real-time PCR) techniques. The genome (the whole of a cell, [...]

Applications in PCR: PCR in gene expression analysis

Nearly all of the cells in an organism carry the same genetic material and so the same collection of genes, but different cells have different roles and so have different profiles of genes active at any one time. Gene expression [...]

Working in real time: A focus on qPCR

There are lots of different types of PCR (polymerase chain reaction), with many different applications, so what’s so special about quantitative PCR (qPCR), also known as real time PCR, RT PCR or qRT-PCR? Measuring in real time Whereas conventional PCR [...]

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