RNA

/Tag:RNA

Polymerase Chain Reaction: The Past, Present and Future

The polymerase chain reaction (PCR), sometimes described as ‘molecular photocopying’, is a laboratory technique that allows researchers and technicians to amplify DNA or RNA from as little as a single strand. The technology was first created in 1983 by Biochemist [...]

Rosalind Franklin

Last week marked the anniversary of the birth of Rosalind Franklin, born 25th July 1920.   Franklin worked as a chemist and x-ray crystallographer, contributing to many groundbreaking developments and greatly enhancing scientific understanding. This post looks back at some [...]

HRM for the Detection of Important Poxviruses

A high resolution melt (HRM) is a powerful, post-PCR, analysis technique used to identify the DNA products present. First of all PCR is performed with an intercalating dye present. These dyes have the unique property of only binding to double-stranded DNA. [...]

New Zika test approved for CDC use

Zika, Dengue and Chikungunya; 3 viruses alike in their symptoms and their transmission by Aedes mosquitoes. Former PCR-based Zika tests were unable to rule out infections with Dengue or Chikungunya. A trioplex real-time RT-PCR assay has now been developed for the [...]

PCR a valuable tool for diagnosing HIV infections

The World Health Organisation estimates that approximately 36.7 million people are living with HIV globally. This epidemic still poses serious public health issues. Diagnosis is a key factor in helping to prevent the disease spread as well as ensuring that [...]

PCR helps to create potential myeloma blood test

Researchers from the NIH, the National Cancer Institute (NCI), and the Dana-Farber Cancer Institute of Harvard Medical School have used the polymerase chain reaction (PCR) to help to create a potential multiple myeloma diagnostic. The results were published in the [...]

Biomarker could predict gastric cancer survival

Chinese researchers have found an RNA biomarker that could predict survival for patients with gastric cancer, according to a paper published in the American Journal of Pathology. Gastric cancer is one of the most common cancers in developing countries. It [...]

Zebrafish and PCR confirm feasibility of high-throughput editing

CRISPR/Cas9 genome editing is widely used as a tool in genetic studies. Researchers looking at zebrafish have combined this technique with the polymerase chain reaction (PCR) to understand more about the functions of genes in humans. The research is published [...]

qPCR can help to diagnose depression

Quantitative PCR has played a part in the development of what could be the first objective test for major depressive disorders (MDD) in adults, and one that could predict who might benefit from cognitive behavioural therapy (CBT). Depression is generally [...]

PCR tracks the bacteria in osteomyelitis

US researchers are looking into the use of the polymerase chain reaction (PCR) to improve the detection of the bacteria causing osteomyelitis, an infection of the bone, which could help in choosing the right antibiotic and monitoring the progress of [...]

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